Nuevas estrategias metodológicas y de análisis de datos de citrometría de flujo aplicadas al diagnóstico y clasificación de las hemopatías malignas

Tesis por compendio de publicaciones[ES] Hoy en día el diágnostico y clasificación de las hemopatías malignas se asienta sobre las características citomorfológicas e histopatológicas del tumor, el inmunofenotipo de las células tumorales y sus características genéticas y moleculares, consideradas en el contexto del comportamiento clínico de la enfermedad

Plain water consumption is associated with lower intake of caloric beverage: cross-sectional study in Mexican adults with low socioeconomic status

Fifteen level single phase multilevel inverter is constructed using power circuit consist of 7 MOSFETs, seven independents DC sources, seven diodes, and an H-Bridge circuit. Switching method used in this MLI is sinusoidal pulse width modulation (SPWM) multicarrier where the reference wave in the form of a sinusoidal wave.  Multicarrier SPWM circuit is designed using electronic components such as Op-Amp that used as a comparator and some logic gates such as AND, OR and NOT gate to trigger S1, S2, S3, S4, S5, S6, S7, and H-Bridge circuit. Carrier waves used in this MLI modified from the triangle wave that having a frequency into carrier waves without frequency (DC sources) where THD that generated into 5.502%. While methods that use frequency such as Phase Disposition PWM (PD PWM), Phase Shift PWM (PS-PWM), and Carrier Overlapping PWM (CO PWM) that produces THD greater than the proposed method, this system was simulated using PSIM software. Abstract — Fifteen level single phase multilevel inverter is constructed using power circuit consist of 7 MOSFETs as switch, 7 indevendent DC sources, 7 diodes, and a H-Bridge. Trigering technic using sinusoidal pulse width modulation (SPWM) with 14 carrier wave, which pure sinusoidal wave as reference, SPWM circuit is disained using Op-Amp as comparator and some logic gates such as AND, OR and NOT gate in order to triggering S1, S2, S3, S4, S5, S6 and S7. By modificated carrier wave from triangular into DC source wave, this MLI produces THD output wave equal to 5.5019684%, while using methodes Phase Disposition PWM (PD PWM), Phase Shift PWM (PS PWM), and Carrier Overlapping PWM (CO PWM) produces THD more than proposed method, this system simulated using PSIM program. Keywords: Multilevel Inverter, SPWM, THD, PSI

La disfunción familiar como factor limitante para lograr un adecuado control glucémico en pacientes diabéticos tipo 2 en el Hospital Rural Pedro Vicente Maldonado desde enero 2010 hasta enero 2012

La DM 2 es un problema de salud pública a nivel mundial, es una de las enfermedades con mayor impacto socio-sanitario, no solo por su alta prevalencia y su crecimiento alarmante, sino también por las complicaciones crónicas; su elevada tasa de mortalidad, el aumento de factores predisponentes y desconocimiento de sus factores de riesgo

Historia del maíz desde tiempos ancestrales hasta la actualidad

Corn is a plant that has been cultivated by humans for thousands of years in America. Currently, it is one of the most important crops in the world, since it is used for both human and animal food. In the Montalvo region, Los Ríos province, Ecuador, the cultivation of maize has been an important economic activity for decades. Local farmers have used traditional farming techniques, such as no-tillage and crop rotation, to produce high-quality maize. In recent decades, with the incorporation of new agricultural technologies, corn production in the region has improved. Farmers have adopted modern cultivation practices, such as the use of improved seeds, fertilizers, and pesticides, which have increased production and improved the quality of maize. In addition, the implementation of irrigation systems has allowed the production of corn throughout the year, which has increased the profitability of the crop. Harvesting and storage of corn has also been improved, which has reduced post-harvest losses. At present, maize continues to be an important crop in the Montalvo region and the Los Ríos province in general. Farmers continue to adopt new technologies to improve the production and quality of corn, which allows them to compete in the national and international markets.El maíz es una planta que ha sido cultivada por los seres humanos durante miles de años en América. En la actualidad, es uno de los cultivos más importantes del mundo, ya que se utiliza tanto para la alimentación humana como para el animal. En la región de Montalvo, provincia de Los Ríos, Ecuador, el cultivo del maíz ha sido una actividad económica importante desde hace décadas. Los agricultores locales han utilizado técnicas tradicionales de cultivo, como la siembra directa y la rotación de cultivos, para producir maíz de alta calidad. En las últimas décadas, con la incorporación de nuevas tecnologías agrícolas, se ha mejorado la producción de maíz en la región. Los agricultores han adoptado prácticas modernas de cultivo, como el uso de semillas mejoradas, fertilizantes y pesticidas, lo que ha permitido aumentar la producción y mejorar la calidad del maíz. Además, la implementación de sistemas de riego ha permitido la producción de maíz durante todo el año, lo que ha aumentado la rentabilidad del cultivo. También se ha mejorado la recolección y almacenamiento del maíz, lo que ha reducido las pérdidas postcosecha. En la actualidad, el maíz sigue siendo un cultivo importante en la región de Montalvo y la provincia de Los Ríos en general. Los agricultores continúan adoptando nuevas tecnologías para mejorar la producción y la calidad del maíz, lo que les permite competir en el mercado nacional e internacional.&nbsp

The proliferation index of specific bone marrow cell compartments from myelodysplastic syndromes is associated with the diagnostic and patient outcome

This is an open-access article distributed under the terms of the Creative Commons Attribution License.-- et al.Myelodysplastic syndromes (MDS) are clonal stem cell disorders which frequently show a hypercellular dysplastic bone marrow (BM) associated with inefficient hematopoiesis and peripheral cytopenias due to increased apoptosis and maturation blockades. Currently, little is known about the role of cell proliferation in compensating for the BM failure syndrome and in determining patient outcome. Here, we analyzed the proliferation index (PI) of different compartments of BM hematopoietic cells in 106 MDS patients compared to both normal/reactive BM (n = 94) and acute myeloid leukemia (AML; n = 30 cases) using multiparameter flow cytometry. Our results show abnormally increased overall BM proliferation profiles in MDS which significantly differ between early/low-risk and advanced/high-risk cases. Early/low-risk patients showed increased proliferation of non-lymphoid CD34 + precursors, maturing neutrophils and nucleated red blood cells (NRBC), while the PI of these compartments of BM precursors progressively fell below normal values towards AML levels in advanced/high-risk MDS. Decreased proliferation of non-lymphoid CD34 + and NRBC precursors was significantly associated with adverse disease features, shorter overall survival (OS) and transformation to AML, both in the whole series and when low- and high-risk MDS patients were separately considered, the PI of NRBC emerging as the most powerful independent predictor for OS and progression to AML. In conclusion, assessment of the PI of NRBC, and potentially also of other compartments of BM precursors (e.g.: myeloid CD34 + HPC), could significantly contribute to a better management of MDS.This work has been partially supported by the following grants: RTICC RD06/0020/0035 - Fondo Europeo de Desarrollo Regional FEDER) - from the Instituto de Salud Carlos III, Ministerio de Economía y Competitividad, Madrid, Spain; M.J.-A. was supported by a grant from the Programa Personal Técnico de Apoyo a la Investigación. Ministerio de Ciencia e Innovación - Universidad de Salamanca, Spain.Peer Reviewe

MRD detection in multiple myeloma: comparison between MSKCC 10-color single-tube and EuroFlow 8-color 2-tube methods

[EN] In patients with multiple myeloma, obtaining posttreatment minimal residual disease (MRD) negativity is associated with longer progression-free survival and overall survival. Here, we compared the diagnostic performance of a single 10-color tube with that of a EuroFlow 8-color 2-tube panel for MRD testing. Bone marrow samples from 41 multiple myeloma patients were tested in parallel using the 2 approaches. Compared with the sum of the cells from the EuroFlow two 8-color tubes, the Memorial Sloan Kettering Cancer Center (MSKCC) single 10-color tube had a slight reduction in total cell number with a mean ratio of 0.85 (range, 0.57-1.46; P < .05), likely attributable to permeabilization of the cells. Percent of plasma cells showed a high degree of concordance (r2 = 0.97) as did normal plasma cells (r2 = 0.96), consistent with no selective plasma cell loss. Importantly, concordant measurement of residual disease burden was seen with abnormal plasma cells (r2 = 0.97). The overall concordance between the 2 tests was 98%. In 1 case, there was a discrepancy near the limit of detection of both tests in favor of the slightly greater theoretical sensitivity of the EuroFlow 8-color 2-tube panel (analytical sensitivity limit of MSKCC single 10-color tube: 6 cells in 1 million with at least 3 million cell acquisitions; EuroFlow 8-color 2-tube panel: 2 cells in 1 million with the recommended 10 million cell acquisitions)

Adaptation of the Bergen Social Media Addiction Scale (BSMAS) in Spanish

The impact of social networks on people's daily lives is worrisome, particularly in adolescents and young people, who seem to exceed the limits of normal use. Constant excessive use can lead to pathological behaviors linked to social media addiction (SMA). Our objectives were to 1) adapt the Bergen Social Media Addiction Scale (BSMAS) to Spanish and 2) evaluate its psychometric properties in a young population. The BSMAS was adapted to Spanish, involving experts on social media addiction and people from the target population during the adaptation process. For the psychometric evaluation, 650 Peruvian college students responded to the Spanish version (53.5 % women aged 18 to 40, M = 21.5 SD = 2.7). The one-dimensional measurement model proposed for the original BSMAS was confirmed for our version (X2(9) = 23.9315, CFI = 0.994, TLI = 0.990, SRMR = 0.032, RMSEA = 0.061). The reliability was good (α = 0.863; 95 % CI: 0.848–0.870; ω = 0.864; 95 % CI: 0.846–0.844), and the measurement invariance was confirmed for sex and age by fitting models. The concurrent validity with external social media addiction and mental health indicators was also confirmed. This study provides new and relevant information on the BSMAS validity and allows its application to Spanish-speaker college students from Peru and similar countries.info:eu-repo/semantics/publishedVersio

Immunophenotype of normal vs. myeloma plasma cells: Toward antibody panel specifications for MRD detection in multiple myeloma

In recent years, several studies on large series of multiple myeloma (MM) patients have demonstrated the clinical utility of flow cytometry monitoring of minimal residual disease (flow-MRD) in bone marrow (BM), for improved assessment of response to therapy and prognostication. However, disturbing levels of variability exist regarding the specific protocols and antibody panels used in individual laboratories. Overall, consensus exists about the utility of combined assessment of CD38 and CD138 for the identification of BM plasma cells (PC); in contrast, more heterogeneous lists of markers are used to further distinguish between normal/reactive PCs and myeloma PCs in the MRD settings. Among the later markers, CD19, CD45, CD27, and CD81, together with CD56, CD117, CD200, and CD307, have emerged as particularly informative; however, no single marker provides enough specificity for clear discrimination between clonal PCs and normal PCs. Accordingly, multivariate analyses of single PCs from large series of normal/reactive vs. myeloma BM samples have shown that combined assessment of CD138 and CD38, together with CD45, CD19, CD56, CD27, CD81, and CD117 would be ideally suited for MRD monitoring in virtually every MM patient. However, the specific antibody clones, fluorochrome conjugates and sources of the individual markers determines its optimal (vs. suboptimal or poor) performance in an eight-color staining. Assessment of clonality, via additional cytoplasmic immunoglobulin (CyIg) κ vs. CyIgλ evaluation, may contribute to further establish the normal/reactive vs. clonal nature of small suspicious PC populations at high sensitivity levels, provided that enough cells are evaluatedGrant sponsor: Red Tematica de Investigacion Cooperativa en Cancer (RTICC) of the Instituto de Salud Carlos III (Ministry of Economy and Competitivity, Madrid, Spain) – FEDER; Grant number: RD12/0036/0048; Grant sponsors: EuroFlow Consortium; the International Myeloma Foundation-Black Swan Research Initiative.Peer Reviewe

Safety and Efficacy of a New Synthetic Material Based on Monetite, Silica Gel, PS-Wallastonite, and a Hydroxyapatite Calcium Deficient: A Randomized Comparative Clinic Trial

Background and Objectives: Maxillary bone defects related to post-extraction alveolar ridge resorption are usual. These defects may lead to failure in further surgical implant phases given the lack of bone volume to perform the dental implant. The objective of this clinical assay was to evaluate the safety and efficacy of an experimental synthetic bone substitute in the preservation of post-extraction maxillary alveoli. Materials and Methods: 33 voluntary patients who had at least one maxillary premolar tooth that was a candidate for exodontia (n = 39) and subsequent implant rehabilitation participated. The regenerated alveoli were monitored by means of periodic clinical examinations (days 9 ± 1, 21 ± 4, 42 ± 6, and 84 ± 6), measuring the height and width of the alveolar crest (days 0 and 180 ± 5), measurement of radiodensity using tomographic techniques (days 0–5 and 175 ± 5), and histological examination of biopsies collected at 180 ± 5 days. Results: No significant differences were observed during the entire follow-up period between the two groups with respect to the safety variables studied. A variation in width of −0.9 ± 1.3 mm and −0.6 ± 1.5 mm, and a variation in height of −0.1 ± 0.9 mm and −0.3 ± 0.7 mm was observed for experimental material Sil-Oss® and Bio-Oss®, respectively. The radiodensity of the alveoli regenerated with the experimental material was significantly lower than that corresponding to Bio-Oss®. However, the histological study showed greater osteoid matrix and replacement of the material with newformed bone in the implanted beds with the experimental material. Conclusions: Both materials can be used safely and proved equally effective in maintaining alveolar flange dimensions, they are also histologically biocompatible, bioactive and osteoconductive. The experimental material showed the advantage of being resorbable and replaced with newformed bone, in addition to promoting bone regeneration

Emotional Literacy in Vulnerable Contexts with Digital Drawings

The objective sought to determine the effects on emotional literacy in the elaboration of digital drawings and traditional drawings in the cooperative and individual pedagogical format. 120 schoolchildren from vulnerable contexts participated. The data was collected by a scale based on Fury's proposals. Se advierten efectos de mejora en el cambio de laalfabetización emocional, y en sus componentes de expresividad y valencia. Improvement effects are noted in the change of emotionalliteracy, and in its components of expressiveness and valence. A higherlevel was detected in the change from negative to positive emotions in thedrawings of children who used tablets collaboratively, as opposed to thosewho did so individually